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columns can be classfied
s.p for GLC :- polyethylene glycol, esters, anides
2. open tubular or capillary column or golay column :-
3. SCOT column (support coated open tubular column)
5. DETECTOR :- decides sensitivity of GC
• Burning of compounds in a flame will lead to ion poduction which are collected at opposite electrodes → current produced is directly proportional to concentration of solute.
• Burning H2 is provided as fuel which is supported by air in the form of pure oxygen.
• C.G + OXYGEN → few electron
• C.G + sample + oxygen → more ions → more electron
• About 90% of drug GC analysis are analysis are analyzed by FID.
6. FLOW METER :- Measure flow of CG limit is 20-60 ml\min. Soap bubble flow meter is most commonly used.
7. MODIFICATION IN GC :-
DERIVATIZATION :- To convert polar to non polar , to increase the thermal stability , to increase the ability of detection, Aid in identification.
Temperature programming :- when the mixture contain compounds of widely differing volatility use of chromatograph in iso thermal condition gives unsatisfactory results.
APPLICATION OF GC :-
3. miscellaneous :-
MCQ
1. Which of the following is not a feature of carrier gas used in gas chromatography?
a) It must be chemically inert
b) It should be suitable for the detector employed
c) It should not be completely pure :
d) It should be cheap
2. In which of the following methods are liquid samples injected into the column in gas chromatography?
a) Gas tight syringe
b) Micro-syringe:
c) Rotary sample valve
d) Solid injection syringes
3. Which of the following is the commonly used support material for the packed column in gas chromatography?
a) Glass
b) Metal
c) Diatomaceous earth:
d) Stainless steel
4. Which of the following is the disadvantage of coiled or helical shaped packed chromatographic column?
a) It cannot be packed uniformly
b) It cannot be repacked easily:
c) It is not compact
d) It is not easy to heat it evenly
5. Which detector is not used in GC ?
a. FID
b. TCD
c.A &B
d. TMD
6.which is not application of GC chromatography?
a.Quality control and analysis of drug products like anti biotics (penicilin), anti viral (amantidine), general anesthetics, etc.
b.polluent like formaldehyde, carbon monoxide , benzene , DDT etc.
c.ASSAY of drugs – purity of a compound can be determined for drugs like :-ATROPINE SULPHATE
d. It is purified water.
answer key
1.c
2.b
3.c
4.b
5.d
6.d
REFERNCE:-
TEXT BOOK OF PHARMACEUTICAL ANALYSIS THIRD EDITION OF DR. RAVI SANKAR (17.1 TO 17.20)
TEXT BOOK OF PHARMACEUTCAL ANALYSIS FOUR EDITION OF DAVID.G.WATSON (261-290)
The post Gas CHROMATOGRAPHY INSTRUMENTATION AND APPLICATION; Questions with Answers appeared first on Gpatindia: Pharmacy Jobs, Admissions, Scholarships, Conference,Grants, Exam Alerts.
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