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]]>In any enzyme catalyzed reaction, the enzyme exist in two forms: free or uncombined form E and combined form ES(enzyme-substrate complex).
The ES complex breaks down and yields product and now the enzyme is free to bind to another substrate.
Enzymatic reactions occur in two stages shown in below equations:
K1, K2 and K3 are rate constant. Michaelis-Menten Equation describes how reaction velocity varies substrate concentration. The mathematical equation is given as:-
Where,
A more convenient procedure to find out the values for Km and Vmax is double-reciprocal plot. The equation is obtained by taking the reciprocal of Michaelis-Menten equation:
When 1/V is plotted against 1/[S], a straight line obtains with a slope equal to Km/Vmax. The point at which line intersects the Y-axis, is equal to 1/Vmax and the point at which line intersects the x-axis is equal to -1/Km.
1. What is enzyme kinetics?
A. Study of [S]
B. Study of rate of reaction
C. Study of initial velocity
D. All of the above
2. What is the effect on initial velocity if the substrate concentration is low?
A. Increases rapidly
B. Increases slowly
C. Decreases
D. Remains constant
3. What is the effect on initial velocity if the substrate concentration is high?
A. Increases rapidly
B. Increases slowly
C. Decreases
D. Remains constant
4. In how many forms, enzymes exists in an enzymatic reaction?
A. 2
B. 3
C. 1
D. 4
5. In which form the enzyme exists when the maximum velocity is observed?
A. E form
B. ES form
C. Both
D. None
6. In which form the enzyme exists when [S] is low?
A. E form
B. ES form
C. Both
D. None
7. When the reaction reaches its plateau state, which type of kinetics is seen?
A. Zero order kinetics
B. First order kinetics
C. Second order kinetics
D. None of the above
8. Match the following-
a. initial velocity 1. [S]
b. Michaelis-Menten constant 2. V0
c. maximum velocity 3. K(M)
d. substrate concentration 4. V(MAX)
9. Which equation is required to determine the mechanism of action of enzyme inhibitors?
A. Michaelis-Menten Equation
B. Lineweaver-Burk plot
C. Double reciprocal plot
D. Both B and C
10. Turnover number is also known as?
A. Km
B. Kcat
C. Kcam
D. Kcad
11. In which condition, V0=Vmax?
A. Low [S]
B. High [S]
C. Low V0
D. High V0
12. Which of the following statement is NOT true?
A. Turnover number is also known as catalytic constant
B. The reaction for the formation of ES is irreversible
C. The reaction is in steady state when rate of synthesis is equal to rate of degradation
D. Plateau is called Vmax
13. Which of the following is the slope of double-reciprocal plot?
A. Km/V0
B. Vmax/Km
C. -1/Km
D. None of the above
14. Which of the following terms are involved in Michaelis-Menten Equation?
A. Vmax
B. Km
C. V0
D. All of the above
15. The point at which the line intersects the y-axis of double-reciprocal plot is numerically equal to?
A. Km/V0
B. Vmax/Km
C. -1/Km
D. 1/Vmax
ANSWERS:-
1. Study of rate of reaction
2. Increases rapidly
3. Increases slowly
4. 2
5. ES form
6. E form
7. Zero order kinetics
8. a – 2 b – 3 c – 4 d – 1
9. Both B and C
10. Kcat
11. Low [S]
12. The reaction for the formation of ES is irreversible
13. None of the above
14. All of the above
15. 1/Vmax
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REFERENCE:- Pankaja Naik- Biochemistry; 4th edition; page no:- 88-90.
The post Enzyme Kinetics and Question Answer for NEET, GPAT, GATE, SSC, Pharmacist, CSIR NET, Nursing Exam appeared first on Gpatindia: Pharmacy Jobs, Admissions, Scholarships, Conference,Grants, Exam Alerts.
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