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]]>Enzyme linked Immunosorbent assay is commonly known as ELISA. where Ag-Ab interaction is monitored by enzyme measurement.
It is similar in principle to RIA but depends on an enzyme rather than a radioactive labels.
An enzyme conjugated with anti body reacts with a colourless substrate is called a chromogenic substrate.
This technique was developed by in 1971 by peter peter Perlman and eva engvall at Stockholm University in Sweden.
PRINCIPLE :-
Principal is based on the formation of Ag-Ab complex which is detected by chromogenic detection using enzyme conjugated secondary antibodies.
The conjugated enzyme act on a specific substrate called chromogenic substrate and generate a color reaction product.
This product is qualitative or quantitative read using an ELISA Plate reader.
TYPES OF ELISA :-
APPLICATION OF ELISA :-
MCQ
1.which is working principle of ELISA ?
A.Ag-Ab neturalization
B.Ag-Ab complex
C. A and B
D.None of the above
2. which is not application of ELISA ?
A.Detection of hepatitis B markers im serum.
B.Percentage of Hb in blood.
C.Detection of HIV antibodies in blood sample.
D.Detection of mycobacterium antibodies in tuberculosis.
3.Indirect ELISA which is detected in sample ?
A.Antigen
B.Anti body
C.A and B
D.None of the above
4.Indirect ELISA which is detected in sample ?
A.Antigen
B.Anti body
C.A and B
D.None of the above
5.sandwich ELISA which is detected in sample?
A.Antigen
B.Anti body
C. A and B
D.None of the above
6.COMPETITIVE ELISA which is detected in sample ?
A.antigen
B.anti body
C.a and b
D.none of the above
ANSWER KEY :-
1.B
2.B
3.A
4.B
5.A
6.B
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REFERENCE:-
Textbook of microbiology by Prescott, Harley.
Practical analytical chemistry by BECKEET.
The post ELISA ( Enzyme linked Immunosorbent): Principle,Procedure,Types and MCQ for NET JRF, GPAT appeared first on Gpatindia: Pharmacy Jobs, Admissions, Scholarships, Conference,Grants, Exam Alerts.
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