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]]>TLC is method of analysis in which the stationary phase is spread as a thin layer on a rigid supporting plate.
The mobile phase a liquid is allowed to migrate across the stationary of plate.
TLC is a solid- liquid form of Chromatography, where the s.p. is polar and m.p. is non polar.
Principle :-
The principle of separation is adsorption.When a mixture containing one or more components are spotted on a TLC of absorbant coated on a chromatographic plate and introduce into a m.p. which are continuing distributing.
The m.p. is flow by capillary action.
The components with more affinity travel slow and that of lesser affinity travel faster.
Advantage of TLC :-
Practical Requirements :-
1. Stationary phase :-
A large number of adsorbent can be used as stationary phase.
Choice of adsorbent:-
MCQ
1. What is the principle of TLC ?
A. Partition
B. Absorption
C. Adsorption
D. A and B
2. Which sentence is false about TLC ?
A. It is very sample method
B. It is less time consuming.
C. Applied to allow type of sample.
D. It is very costly.
3. Which is not factor for choosing adsorbent ?
A. Nature of the adsorbent
B. Solubility of the adsorbent
C. Chemical nature
D. Temperature.
4. The m.p. is flow by
A. Capillary action
B. Partition coefficient
C. Temperature
D. All of the above
5. The components with more affinity towards the stationary phases is
A. Travels slow
B. Travels fast
C. A and B
D. None of the above
6.The components with less affinity towards the stationary phases is
A. Travels slow
B. Travels fast
C. A and B
D. None of the above
Answer key
1. C
2. D
3. D
4. A
5. A
6. B
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The post Thin layer Chromatography: Principle, Procedure, Experimental Requirement, Advantage and Question Answer appeared first on Gpatindia: Pharmacy Jobs, Admissions, Scholarships, Conference,Grants, Exam Alerts.
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