Chromatography: Classification, Basic Terms and MCQ with Answers

Chromatography :-

Definition :- chromatography is the method of separation of components from complex mixture by a continuous distribution of the compenent between two phase.

Two phase are 1. Stationary phases 2. Mobile phase ( moving on stationary phase)

Chromatography meaning is colour writing.

Force that involve in chromatography :-

  • Electrostatic force
  • Hydrogen bonding force
  • London force

Basic term of Chromatography :-

  •  Analyte :- substance for separation.
  • Elution :- is a process of removing adsorbed material from stationary phase by the movement of mobile phase.
  • Eluent :- It is a solvent that used for  separation of absorbed material from stationary phase.
  • Eluate :- is a liquid solution that is a result from Elution.
  • Chromatogram :- It is a graphical represention of Chromatography. Solute concentration vs Elution time ot Elution volume.
  • Counter current chromatography ( ccc) :- is a type of liquid- liquid Chromatography where m.p. and s.p both are liquid.
  • Polar compound :- A compound that hold by stationary phase
  • Non polar compound :- A compound moving by Mobile phase.
  • Reversed phase is only chromatography that m.p. has more polar compound than stationary phase.
  • Retention Time :- Time required to elute one half of the compound from the column as indicated by peak maximum.
  • Retention volume :- volume of m.p. required to elute one half of the compound from the column as indicated by peak maximum.
  • Non Hazardous substitution for RIA is HPLC.

Classification of Chromatography :-

1. Classification based on Method geometry or type of separation bed used :-

A. Planar chromatography :- paper and thin layer Chromatography

B. Column chromatography :- GC, HPLC and SFC

2. Classified based on type of mobile phase or stationary phase

A. Stationary phase :- HPLC and GLC

B. Stationary phase :- GSC

3. Classification according to the mechanism of separation :-

A. Adsorption chromatography :- GSC

B. Partition Chromatography :- liquid liquid Chromatography

C. Affinity chromatography

D. Ion exchange chromatography

E. Molecular exclusion chromatography.

MCQ

1. In which Chromatography s.p. is more polar than m.p. ?

A. Ion exchange

B. Liquid liquid Chromatography

C. Reversed chromatography

D. None of the above

2. Which force is involved in the Chromatography ?

A. Hydrogen bonding

B. London force

C. Electric static force

D. All of the above

3. Which technique is also known as colour writing ?

A. NMR

B. Mass spectroscopy

C. Chromatography

D. All of the above

4. Non Hazardous substitution for RIA is

A. Uv

B. HPLC

C. NMR

D. None of the above

5. Chromatogram is?

A. Solute concentration vs Elution time

B. Solute concentration vs Elution volume

C. A and B

D. None of the above

6. Which compound are hold by stationary phase ?

A. Polar compound

B. Nom polar compound

C. A and B

D. None of the above

7. What is the Analyte ?

A. Substance for separation

B. Substance for impurity

C. A and B

D. None of the above

8. What is Eluent ?

A. is a liquid solution .

B.is a liquid solution that is a result from Elution.

C. It is a solvent that used for  separation of absorbed material from stationary phase.

D. None of the above

Answer key

1. C

2. D

3. C

4. B

5. C

6. A

7. A

8. C

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