Nephelometry & Turbidimetry: Instrumentation, Principal & MCQ with Answers

Nephelometry :-

It is deals with analysis of colloidal system. It is based on the scattering of light by particle suspended in a liquid.

Principal :-

  • Nephelometry is concerned with measure of the intensity of scattered light as a function of concentration of the suspended particle in a suspension.
  • Intensity of scattered light is directly proportional to the concentration of the suspended particle.
  • Hence greater concentration of particle more the intensity of light.
  • The intensity of the scattered light is usually measure at 90° to the incident light.

Turbidimetry :-

It deal with analysis of colloidal system and the technique is based on transmission of light by particle suspended in a liquid.

Principal :-

  • Turbidimetry is concerned with the measure of the intensity of the transmitted light as a function of concentration of the suspended particle in a suspension.
  • The intensity of transmitted light is measured in a line i.e 180° to the incident light.
  • Intensity of  transmitted light is inversely proportional to the concentration of the suspended particle
  • Hence concentration is more transmission is less.

Instrumentation of Nephelometry & Turbidimetry :-

1. Radiation source :- The common radiation source is used as tungsten filament.

2. Monochromator :- usually prisms or grating monochromator are used.

3. Detector :- mostly Photomultiplier tube are use.

4. Filter :- There are two types of filter are used

A. Absorption filter

B. Interference filter

5. Read out Device :- micro computer are used as read out device.

MCQ

1. What is principal of Nephelometry ?

A. Light scattered

B. Light transmitted

C.A and B

D. None of the above

2. What is principal of Turbidimetry?

A. Light scattered

B. Light transmitted

C.A and B

D. None of the above

3.The intensity of the scattered light is usually measure at which angle?

A. 90°

B. 44°

C. 60°

D. 70°

4. The intensity of the transmitted light is usually measure at which angle?

A. 80°

B. 180°

C. 90°

D. 100°

5. Which technique is used to analyze colloidal system?

A. Nephelometry

B. Turbidimetry

C. A and B

D. None of the above

6. Which sentence is false about Nephelometry ?

A. Nephelometry is concerned with the measure of the intensity of the transmitted light as a function of concentration of the suspended particle in a suspension.

B.Intensity of scattered light is directly proportional to the concentration of the suspended particle.

C.Hence greater concentration of particle more the intensity of light

D.The intensity of the scattered light is usually measure at 90° to the incident light.

7. Which sentence is false about Turbidimetry ?

A.Turbidimetry is concerned with the measure of the intensity of the transmitted light as a function of concentration of the suspended particle in a suspension.

B.The intensity of transmitted light is measured in a line i.e 180° to the incident light.

C.Hence concentration is more transmission is less.

D.Hence greater concentration of particle more the intensity of light.

Answer key :-

1. A

2. B

3. A

4. B

5. C

6. A

7. D

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