Production of Plant Secondary Metabolites: MCQ for DBT BET, GPAT, GATE, & CSIR NET
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Advantages for production of secondary metabolites
- Production of compounds can be done in controlled conditions as per market demand.
- Environmental factors, seasonal variations, pest, and microbial diseases, and geographical constraints can be avoided by the in-vitro cultural system
- Product formation can be facilitated by control over cell growth.
- The consistency of quality products can be achieved as they are produced from a specific cell line.
- Product recovery is easy
- In-vitro cultures are important when plants are expensive or difficult to grow in fields.
- Mutant cell lines can be developed for the production of novel compounds.
- Plant tissue culture techniques can minimize time and labor cost
Disadvantages
- In compared to intact plants, production of secondary metabolites is lower in Invitro culture
- There are chances of cell damage during culture due to vigorous stirring
- Strict aseptic conditions have to be maintained, otherwise, any infection to the culture can affect production.
Examples of secondary metabolites used as drugs
Secondary metabolite (drug) |
Use |
Vincristine |
Anti-cancer |
Podophyllotoxin |
Anti-neoplastic |
vinblastine |
Anti-neoplastic |
digoxin |
Cardiotonic |
Morphine |
Analgesic |
Codeine |
Analgesic |
Quinine |
Anti-malarial |
Nicotine |
Insecticidal |
Sennosoides (Anthraquinone) |
Laxatives |
Multiple-choice questions
1._____________is the source for Production of secondary metabolite a) Primary metabolite b) DNA c) RNA d) Carbohydrates 2. Secondary metabolites are used by plant cells for__________ a) Production of nucleic acids b) For making plasma membrane c) Morphological differentiation d) All 3. Secondary metabolite is source for ____________ a) Drugs b) Fragrances c) Dyes d) All 4. Which one of the following is NOT secondary metabolite a) Proteins b) Alkaloids c) Terpenes d) Terpenoids 5. _______________is one of the function of secondary metabolite a) As drug b) To attract birds c) To make disease free plant d) all 6. _______________is visually identified secondary metabolite a) Digitoxin b) Digoxin c) Beta carotene d) Quinine 7. Elicitors are the compounds used to enhance the growth, size, and shape of plant cells in tissue culture a) True b) False c) Partially True d) None of the above 8. ___________is Endogenous type of elicitor a) Temperature b) Pectin c) Chitin d) Fungicide 9. __________ range of temperature is required for in-vitro culture a) 200 to 250 b) 300 to 350 c) 250 to 300 d) 350 to 400 10. Production of secondary metabolites by plant tissue culture technique is preferred because a) Production yield is very high b) Product recovery is easy c) Aseptic conditions can be easily maintained d) No skilled person is required Answer Key- a
- b
- d
- a
- a
- c
- b
- b
- c
- b
Reference
- U. Satyanarayana, biotechnology, 1st e.d. Kolkata, books & allied (p) ltd publishers, 2005 page:507-516