SPECTRO FLUORIMETRY Basics and Principle: Question with Answers

Fluorimetry is analytical method which is based on the absorbed or emitted radiation by the molecule. FLUORESCENCE :- when molecule absorbed incident electromagnetic radiation it is electron goes excited state. But in excited state it is unsatble so. Electron is […]

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Factor that affecting Fluorescence and Quenching and MCQ asked in EXAM

Factor that affecting fluorescence :- 1. Nature of the molecule :- Molecules that have tendency to absorbed  Electromagnetic radiation (uv,IR) can only exhibit fluroscence. Unsaturated molecules with pi(π) bond and good resonance that molecules exhibit fluroscence. Saturated molecules with sigma(σ) bond  do […]

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