PRINCIPLE:- It is based on the principle of polarography , with the exception that the voltage is maintained constant during the titration. Diffusion current ∝ concentration of electroredusible ion Potential applied between polarizable & non polarizable is kept constant. INSTRUMENTATION:- Dropping […]
PROCEDURE OF HPLC:- The sample is introduced through an injection system into the entrance to the column. The stationary phase is porous silica. mobile phase can either be aqueous or organic solvent In interaction between mobile and stationary phase, two […]
INTODUCTION :- High perfomance liquid chromatography is a specific form of a column chromatography generally used in bio chemistry and analysis to separate, identify and quantify the active compound. PRINCIPLE :- HPLC is principle is based on adsorption as well […]
Column characteristics :- The length: diameter ratio of column is 10:1 to 30:1. The length of the column is depend upon Affinity of compound towards the adsorbent used. Number of compound to be separated. Type of adsorbent used. Quantity of […]
Introduction :- Column Chromatography is a method of analysis in which the stationary phase is allowed to migrate across the surface of the column. The column is simply a glass tube with a stopcock at the bottom. The column is […]
Cell is a device which convert chemical energy into electrical energy. cell is consists of two half cell. ELECTRODE POTENTIAL:– Difference between the potential of two electrodes. POTENTIOMETRY consists of measuring potential or emf of solution using set of indicator & […]
Paper Chromatography :- Definition :- it is defined as a separation technique where separation of a mixture of compound is mainly achieved by a flow of solvent on a chromatographic paper. Stationary phase has a bound or abosorbed water present […]
Ion- Exchange Technique :- The required components can separated generally by two types of technique. They are 1. Batch Method :- This is a single step method in which the resin and the solution are takes into a single container […]
Ion Exchange Chromatography- Part 2 Properties of Ion exchangers :- The common property of all ion exchanger are as follows :- They are insoluble in water and organic solvent like carbon tetrachloride, benzene, chloroform etc. They are complex in nature. […]
Introduction :- In IEC resin is used to co valently attach anion or cation into it. Analyte ions with opposite charge in the liquid mobile phase are attached to the resin by electrostatic force. Most of the bio molecules and […]