VALIDATION OF UV SPECTROPHOTOMETER INSTRUMENT: PROCEDURE & TEST
VALIDATION OF UV INSTRUMENT :- Performance test 1. Wavelength accuracy :- Standard :- 4% holmium oxide in 10% perchloric acid. Specification :- NMT _+ 1 nm in […]
Chromophore, Auxochrome and different type of shift in UV Spectroscopy: Free Notes & MCQ
Chromophore It is a covalently unsaturated group which is responsible for absorption of UV or visible radiation and may or may not impact in colour to the compound. A compound which contains chromophore it is called chromogen. In unsaturated linkage […]
Fluorimetry: Advantage, limitation and Application
ADVANTAGE Sensitivity :- it is give accurate result even sample is present in microgram concentration in solution. Precision :- upto 1 % can be achieved easily. Specificity :- This method is more specific than absorption method. Range of Application :- […]
NUMBER OF SIGNAL, MECHANISM OF SPLITTING AND SPIN-SPIN COUPLING IN NMR
NUMBER OF SIGNAL IN NMR :- The number of signal in the NMR spectrum give the information about proton(hydrogen) arrangement in a molecule. Each signal of NMR give information about each proton set. Molecules give 1 nmr signal like benzene, […]
NMR SPECTROSCOPY : Basic Terms and Defination
Basic terms of NMR SPECTROSCOPY 1. Proton :- IT is a positive charge particle that present in nuclei of any molecule. 2. NMR SPECTROSCOPY :- when EMR are applied in any sample in presence of magnetic field. So that sample […]