Electro analytical methods are concerned with the interplay between electrictiy and chemistry, namely the measurements of electrical quantities, such as current, potential, or/and their relationship to chemical parameters. If electrical parameter : E-Potentionmetry , R-conductometry, I- voltametry,coulometry MAIN BRANCHES OF ELECTROANALYTICAL […]
PRINCIPLE:- It is based on the principle of polarography , with the exception that the voltage is maintained constant during the titration. Diffusion current ∝ concentration of electroredusible ion Potential applied between polarizable & non polarizable is kept constant. INSTRUMENTATION:- Dropping […]
PROCEDURE OF HPLC:- The sample is introduced through an injection system into the entrance to the column. The stationary phase is porous silica. mobile phase can either be aqueous or organic solvent In interaction between mobile and stationary phase, two […]
INSTRUMENTATION OF HPLC :- HPLC INSTRUMENT CONSISTS OF FOLLOWING COMPONENTS:- PUMP MIXING UNIT SOLVENT DELIVERY SYSTEM INJECTOR COLUMN DETECTOR PUMP:- The role of the pump is to force a liquid through the liquid chromatography at a specific flow rate expressed […]
INTODUCTION :- High perfomance liquid chromatography is a specific form of a column chromatography generally used in bio chemistry and analysis to separate, identify and quantify the active compound. PRINCIPLE :- HPLC is principle is based on adsorption as well […]
Column characteristics :- The length: diameter ratio of column is 10:1 to 30:1. The length of the column is depend upon Affinity of compound towards the adsorbent used. Number of compound to be separated. Type of adsorbent used. Quantity of […]
Introduction :- Column Chromatography is a method of analysis in which the stationary phase is allowed to migrate across the surface of the column. The column is simply a glass tube with a stopcock at the bottom. The column is […]
Introduction :- In IEC resin is used to co valently attach anion or cation into it. Analyte ions with opposite charge in the liquid mobile phase are attached to the resin by electrostatic force. Most of the bio molecules and […]
Formation of urine takes place in kidneys and it is transferred to the bladder before it is excreted from the body. Urine is formed for various reasons such as excretion of waste product of protein metabolism, water and electrolyte levels […]
Thyroid gland The thyroid gland is situated in the neck just in front of larynx and trachea, it is a highly vascular gland of about 25g and is covered or surrounded by a fibrous capsule. It has a butterfly shape […]