INSTRUMANTION OF GAS CHROMATOGRAPHY :- carrier gas :- Transmits the sample form the point of introduction through the column to the detector. examples of carrier gas is hydrogen, helium, or nitrogen flow rate is depends upon column i.d.→2mm/min, i.d.→15-20mm/min purification […]
Introduction :- Gas Chromatography is an analytical technique use for separation of thermally stable and volatile substance. In Gas Chromatography the mobile phase is gas and the stationary phase is solid or liquid. Principle :- The principle involved in gas […]
GPAT 2012 MCQ 1. Quanfication of minute quanity of a drug from a complex matrix, without prior separation can be done using one of the following technique . identify that a. coulometry b. potentiometry c.fluorescence spectroscopy d. radio immunoassay 2. […]
MCQ OF GATE 2008 1.In NMR spectrometry the chemical shift is expressed in a. Parts per million b. Gauss c. Tesla d. HERTZ 2. In chromatographic separation the different species in the sample undergo the process of a.chemical interaction b. […]
There are mainly two theory for column Chromatography is 1. Plate Theory 2. Rate Theory Plate Theory :- Plate Theory is given by martin and synge in 1941. Plate Theory is useful for understanding the mechanism of retention and transfer […]
Paper Chromatography Visualizing Agent :- After development of chromatogram the spot should be visualized. It can done by two Method A. Non specific method B. Specific method A. Non specific method :- where the number of spot can be detected but […]
ADVANTAGE Sensitivity :- it is give accurate result even sample is present in microgram concentration in solution. Precision :- upto 1 % can be achieved easily. Specificity :- This method is more specific than absorption method. Range of Application :- […]
Fluorimetry is analytical method which is based on the absorbed or emitted radiation by the molecule. FLUORESCENCE :- when molecule absorbed incident electromagnetic radiation it is electron goes excited state. But in excited state it is unsatble so. Electron is […]
Diazotization Titration :- Diazotization Titration are used to determination of primary aromatic amine compound. Principle :- Reaction are performed in ice both at temp. 0-5 c. End point is determined by the starch iodine paper or by potentiometric method. Mechanism […]